Human Test Kit (ELISA) for Testing Hepatitis E Virus

Model Number: Anti-HEV IgG ELISA01 Brand Name: Egens Key Specifications/Special Features: This Anti-HEV IgG is a qualitative enzyme immunoassay for the in vitro detection of IgG antibody to Hepatitis EVirus in human serum or plasma. It is intended for diagnosing patients related to infection with HEV.ASSAY PROCEDURE1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer (20×)with 19 volumes of distilled water,mix well.2. Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negativecontrol, 2 wells as positive control. After dispensing 100L of Sample Diluent , dispense 10L of sample,100L negative control or positive control to the respective wells(without Sample Diluent). Gently vibrating theplate. 3. Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlledwater-bath or microplate incubator at 37℃ for 30 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted
washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure
that the rest volume is minimal, by tapping plate onto absorbent paper.
5. Add Conjugate: Add 100L of conjugate to each well (except the blank well).
6. Incubate: Cover the Microplate and incubate the plate at 37℃ for 20 minutes.
7. Wash the Plate: Repeat the wash procedure as in step 4.
8. Add Substrate: Add 50L of Substrate Solution A and 50L of Substrate Solution B to each well, mix well.
Cover and incubate at 37℃ for 10 minutes.
9. Stop reaction: Add 50L Stop Solution to each well, mix well.
10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should
be selected from 620nm to 690nm.